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Reference type: Journal
Authors: Frasconi M, Tel-Vered R, Riskin M, Willner I
Article Title: Surface Plasmon Resonance Analysis of Antibiotics Using Imprinted Boronic Acid-Functionalized Au Nanoparticle Composites.
Publication date: 2010
Journal: Analytical Chemistry
Volume: 82
Issue: (6)
Page numbers: 2512-2519.
DOI: 10.1021/ac902944k

Abstract: Au nanoparticles (NPs) are functionalized with thioaniline electropolymerizable groups and (mercaptophenyl)boronic acid. The antibiotic substrates neomycin (NE), kanamycin (KA), and streptomycin (ST) include vicinal diol functionalities and, thus, bind to the boronic acid ligands. The electropolymerization of the functionalized Au NPs in the presence of NE, KA, or ST onto Au surfaces yields bisaniline-cross-linked Au NP composites that, after removal of the ligated antibiotics, provide molecularly imprinted matrixes which reveal high sensitivities toward the sensing of the imprinted antibiotic analytes (detection limits for analyzing NE, KA, and ST correspond to 2.00 0.21 pM, 1.00 0.10 pM, and 200 30 fM, respectively). The antibiotics are sensed by surface plasmon resonance (SPR) spectroscopy, where the coupling between the localized plasmon of the NPs and the surface plasmon wave associated with the Au surface is implemented to amplify the SPR responses. The imprinted Au NP composites are, then, used to analyze the antibiotics in milk samples
Template and target information: neomycin, NE, kanamycin, KA, streptomycin, ST


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