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Reference type: Journal
Authors: Zhang ZH, Hu YF, Zhang HB, Luo LJ, Yao SZ
Article Title: Layer-by-layer assembly sensitive electrochemical sensor for selectively probing l-histidine based on molecular imprinting sol-gel at functionalized indium tin oxide electrode.
Publication date: 2010
Journal: Biosensors and Bioelectronics
Volume: 26
Issue: (2)
Page numbers: 696-702.
DOI: 10.1016/j.bios.2010.06.062
Alternative URL: http://www.sciencedirect.com/science/article/B6TFC-50G0654-6/2/6248d9afc2225270f7d252b8c4987b51

Abstract: A novel sensitive and selective imprinted electrochemical sensor was successfully constructed for the direct detection of l-histidine by combination of a molecular imprinting film and multi-walled carbon nanotubes (MWNTs). The sensor was fabricated onto an indium tin oxide (ITO) electrode via stepwise modification of MWNTs and a thin film of molecularly imprinted polymers (MIPs) via sol-gel technology. The introduced MWNTs exhibited noticeable enhancement on the sensitivity of the MIPs sensor. Meanwhile, the molecularly imprinted film displayed high sensitivity and excellent selectivity for the target molecule l-histidine. The proposed imprinted sensor was characterized by using scanning electron microscope (SEM) and electrochemical methods involving cyclic voltammetry (CV), differential pulse voltammetry (DPV) and amperometric i-t curve. A linear ranging from 2.0 μmol L-1 to 1.0 mmol L-1 for the detection of l-histidine was observed with the detection limit of 5.8 x 10-9 mol L-1 for S/N = 3. This imprinted electrochemical sensor was successfully employed to detect l-histidine in human blood serum
Template and target information: l-histidine
Author keywords: Molecularly imprinted polymers, Electrochemical sensor, Multi-walled carbon nanotubes, Layer-by-layer assembly, L-Histidine


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