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Reference type: Journal
Authors: Sener G, Ozgur E, Yilmaz E, Uzun L, Say RI, Denizli A
Article Title: Quartz crystal microbalance based nanosensor for lysozyme detection with lysozyme imprinted nanoparticles.
Publication date: 2010
Journal: Biosensors and Bioelectronics
Volume: 26
Issue: (2)
Page numbers: 815-821.
DOI: 10.1016/j.bios.2010.06.003
Alternative URL: http://www.sciencedirect.com/science/article/B6TFC-508X3B5-1/2/9bfd27b2ca58ae9bc453ac7840ad886a

Abstract: The aim of this study is to prepare quartz crystal microbalance (QCM) nanosensor for the real-time detection of lysozyme. In the first part, the lysozyme imprinted (MIP) nanoparticles were prepared by mini-emulsion polymerization. The MIP nanoparticles were characterized by TEM, zeta-sizer and FTIR-ATR measurements. Particle size was found around 50 nm. The MIP nanoparticles were attached by dropping of nanoparticle solution to gold surface and then, dried at 37 C for 6 h. QCM nanosensor was characterized with AFM and ellipsometer. The observations indicated that the nanoparticle film was almost monolayer. The detection limit was found as 1.2 ng/mL. The specificity of the QCM nanosensor was shown by using albumin as a competitor molecule. The results show that the QCM nanosensor has high selectivity and sensitivity with a wide range of lysozyme concentrations in both aqueous solutions (0.2-1500 μg/mL) and natural sources (egg white) (460-1500 ng/mL)
Template and target information: protein, lysozyme
Author keywords: QCM, nanoparticles, lysozyme, molecular imprinting, Nanosensors


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