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Reference type: Journal
Authors: Tang JS, Xiang L
Article Title: Development of a competitive format sorbent assay for the determination of parathion in water using molecular imprinted polymer as specific sorbent carrier.
Publication date: 2010
Journal: Chinese Chemical Letters
Volume: 21
Issue: (11)
Page numbers: 1361-1365.
DOI: 10.1016/j.cclet.2010.05.014
Alternative URL: http://www.sciencedirect.com/science/article/B8G3X-511BFJ1-6/2/e4ba9de4d9a23cc9efd1812ea452fc59

Abstract: A rapid, simple, and reliable competitive molecular imprinted polymer sorbent assay (MIPSA) was developed and validated for measurement of parathion in water samples. This assay employed a molecular imprinted polymer (MIP) that was synthesized with non-covalent imprinting method as capture reagent and p-aminoparathion conjugate of horseradish peroxidase (para-HRP) as an enzyme label. The assay depended on a competitive binding reaction between the enzyme conjugate and analyte for the binding sites of the MIP. The optimized analysis conditions of 10 ng mL-1 para-HRP and 10 mg mL-1 polymer were found. The assay was acceptable to detect parathion in water samples under the optimized conditions, with a limit of detection of 50 ng mL-1. Mean analytical recoveries of added parathion in water samples ranged from 101.2% to 105%. The precision of the assay was satisfactory; relative standard deviation ranged from 4.3% to 6%
Template and target information: parathion, p-aminoparathion conjugate of horseradish peroxidase, para-HRP
Author keywords: molecular imprinting, Conjugated enzyme, sorbent assay, Parathion, Water sample


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