MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Xu WJ, Su SF, Jiang P, Wang HS, Dong XC, Zhang M
Article Title: Determination of sulfonamides in bovine milk with column-switching high performance liquid chromatography using surface imprinted silica with hydrophilic external layer as restricted access and selective extraction material.
Publication date: 2010
Journal: Journal of Chromatography A
Volume: 1217
Issue: (46)
Page numbers: 7198-7207.
DOI: 10.1016/j.chroma.2010.09.035
Alternative URL: http://www.sciencedirect.com/science/article/B6TG8-5125R58-1/2/7ed6cc0fe338ad332e0143cf34a276d1

Abstract: A novel restricted access-molecularly imprinted material (RA-MIP) with selectivity for sulfonamides was synthesized using initiator-transfer agent-terminator (iniferter) method, a "living"/controlled radical polymerization technique. The material was prepared by grafting two layers with different functions on the silica support. To perform a "grafting from" polymerization, iniferter was immobilized on the surface of silica. The internal sulfamethazine imprinted polymer and the external poly(glycidyl methacrylate) [poly(GMA)] were then grafted successively. The hydrophilic structures were formed on the external layer of the material by the hydrolysis of the linear poly(GMA) for protein removal. The result has shown that this restricted access-MIP grafted silica (RA-MIP-SG) not only has the selectivity for the template and its analog, but also has the ability of exclusion for bovine serum albumin (BSA). It indicated that the material possesses both properties of molecularly imprinted polymer (MIP) and restricted access material (RAM). Using RA-MIP-SG as pre-column, a column-switching HPLC method was established for the determination of sulfonamides in bovine milk. Direct sample injection was performed in the analysis, which provides a convenient analytical procedure. Good linearity in the range of 2-400 ng mL-1 (R > 0.998) has been obtained for seven sulfonamides in the study. The recoveries of all the analytes at three concentration levels are between 93% and 107% with the RSD < 8.0%. The limits of quantification and limits of detection are less than or equal to 2.7 ng mL-1 and 0.8 ng mL-1 respectively. It demonstrated this RA-MIP-SG can be applied in sample extraction and clean-up for the determination of sulfonamides in bovine milk by direct injection and column-switching HPLC with good efficiency and reliability
Template and target information: sulfamethazine
Author keywords: molecularly imprinted polymer, Restricted access material, Iniferter technique, sulfonamides, Column-switching HPLC, Bovine milk


  I love MIPs apron  multi MIPs logo mug  British periodic table patriotic tote bag

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner