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Reference type: Journal
Authors: Prasad BB, Madhuri R, Tiwari MP, Sharma PS
Article Title: Imprinting molecular recognition sites on multiwalled carbon nanotubes surface for electrochemical detection of insulin in real samples.
Publication date: 2010
Journal: Electrochimica Acta
Volume: 55
Issue: (28)
Page numbers: 9146-9156.
DOI: 10.1016/j.electacta.2010.09.008
Alternative URL: http://www.sciencedirect.com/science/article/B6TG0-511G1VY-3/2/06f8b4899feeb3782d45078bde09bfea

Abstract: An insulin-imprinted polymer was synthesized over the surface of vinyl group functionalized multiwalled carbon nanotubes, using phosphotidylcholine-containing functional monomer and cross-linker. Phosphotidylcholine is a major component of all biological membrane; its incorporation in polymer backbone assures water-compatibility, bio-compatibility and specificity to molecularly imprinted nanomaterials, without any cross-reactivity or interferences from biological sample matrices. An electrochemical sensor fabricated by modifying multiwalled carbon nanotubes-molecularly imprinted polymer onto the pencil graphite electrode, was used for trace level detection of insulin in aqueous, blood serum, and pharmaceutical samples (detection limit 0.0186 nmol L-1, S/N = 3), by differential pulse anodic stripping voltammetry. Additional cyclic voltammetry (stripping mode) and chronocoulometry experiments were performed to explore electrodics and kinetics of electro-oxidation of insulin
Template and target information: peptide, insulin
Author keywords: Insulin-imprinted polymer, MWCNTs-MIP, Carbon-MIP modified PGE, Differential pulse anodic stripping voltammetry


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