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Abstract: An electrochemical sensor for theophylline (ThPh) was prepared by electropolymerizing o-phenylenediamine on a glassy carbon electrode in the presence of ThPh via cyclic voltammetry, followed by deposition of gold nanoparticles using a potentiostatic method. The effects of pH, ratio between template molecule and monomer, number of cycles for electropolymerization, and of the solution for extraction were optimized. The current of the electro-active model system hexacyanoferrate(III) and hexacyanoferrate(IV) decreased linearly with successive addition of ThPh in the concentration range between 4.0 x 10-7 ~ 1.5 x 10-5 mol ·L-1 and 2.4 x 10-4 ~ 3.4 x 10-3 mol ·L-1, with a detection limit of 1.0 x 10-7mol·L-1. The sensor has an excellent recognition capability for ThPh compared to structurally related molecules, can be regenerated and is stable
Template and target information: theophylline, ThPh
Author keywords: molecular imprinted polymer, Electrochemical sensor, Au nanoparticles, theophylline, Recognition