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Reference type: Journal
Authors: Gai QQ, Qu F, Zhang YK
Article Title: The Preparation of BHb-Molecularly Imprinted Gel Polymers and Its Selectivity Comparison to BHb and BSA.
Publication date: 2010
Journal: Separation Science and Technology
Volume: 45
Issue: (16)
Page numbers: 2394-2399.
DOI: 10.1080/01496395.2010.484409
Alternative URL: http://www.informaworld.com/10.1080/01496395.2010.484409

Abstract: Molecularly imprinted gel polymer (MIP) for the selective imprinting of bovine hemoglobin (BHb) was prepared in aqueous media by bulk polymerization using polyacrylamide matrix. The synthesis conditions of BHb-MIP were investigated, which involved the interaction of functional monomers and template protein in different molar ratio, solution pH, and ionic strength. The adsorption experiments indicated that BHb-MIP had a high affinity for BHb over the non-imprinted polymers. The selectivity of BHb-MIP for BHb and bovine serum albumin (BSA) with similar molecular weight was compared. It was demonstrated that BHb-MIP had better selective adsorption and recognition properties to BHb especially in the presence of BSA as competing protein. It might be helpful for selectively separating template protein with MIP from the proteins mixture with similar molecular weight
Template and target information: protein, bovine hemoglobin, BHb
Author keywords: Bovine hemoglobin, Bovine serum albumin, molecularly imprinted gel polymer


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