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Abstract: The automated method was developed for the determination of low mol. wt. compounds in biological fluids. Samples are fractionated on a restricted-access column, the analyte fraction is then applied to a column of a molecularly imprinted polymer and the retained compound is eluted either for direct detection or for further HPLC separation. The procedure was exemplified by the determination of tramadol in blood plasma. Samples (0.1 ml) were applied to a column (2.5 cm × 4 mm i.d.) of LiChrospher ADS RP18, which was then washed with acetonitrile/0.1% triethylamine adjusted to a pH of 7 with TCA (1:19) at 0.8 ml/min for 10 min. The tramadol was transferred with acetonitrile at 0.8 ml/min for 5 min to the imprinted polymer column (2.5 cm × 4 mm i.d., prepn. described), with further washing with acetonitrile for 3 min, then the tramadol was eluted with the mobile phase (0.8 ml/min) of acetonitrile/0.1% triethylamine adjusted to a pH of 2.6 with TCA (1:3) on to the analytical column (12.5 cm × 4 mm i.d.) of 5 μm LiChrospher CN at 50°C, with detection at 220 nm. The method was named Six-S ProcEdure (size-selective sample separation and solvent switch). (Analytical Abstracts 2000)
Template and target information: tramadol