MIPs logo MIPdatabase      MIP2018 Conference website
Custom Search
Reference type: Journal
Authors: Levi R, McNiven S, Piletsky SA, Cheong SH, Yano K, Karube I
Article Title: Optical detection of chloramphenicol using molecularly imprinted polymers.
Publication date: 1997
Journal: Analytical Chemistry
Volume: 69
Issue: (11)
Page numbers: 2017-2021.
DOI: 10.1021/ac960983b

Abstract: A practical optical sensing system for the determination of chloramphenicol (CAP), utilizing molecularly imprinted polymers (MIPs) and HPLC, has been developed, The method is based on competitive displacement of a chloramphenicol-methyl red (CAP-MR) dye conjugate from specific binding cavities in an imprinted polymer by the analyte. The best of these polymers was obtained using (diethylamino)ethyl methacrylate as functional monomer at a monomer:template ratio of 2:1. HPLC with a mobile phase containing CAP-MR was used as the detection system, and injection of CAP and, to a lesser degree, thiamphenicol resulted in proportional displacement of the conjugate, which was detected at 460 nn, The detection system showed a linear response over a range of 3 - 1000 μg/mL and effectively detected CAP extracted from serum, This system offers a tailor-made, selective, and rapid method for CAP detection, is able to discriminate between similar molecules, and is effective below and above the therapeutic range (10-20 μg/mL serum, potentially toxic above 25 μg/mL). This technique is quite general and should enable the use of MIPs in a wide variety of applications involving the detection of families of molecules which possess a distinct arrangement of functional groups


  Shirt featuring the name Sergey spelled out in symbols of the chemical elements  multi MIPs logo mug  American periodic table patriotic shirt

Molecules Special Issue call






Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:
US listings   UK listings

Searching for Molecular Imprinting books on Amazon.com:
Molecular Imprinting
Imprinted Polymers
Polymer Chemistry
Organic Chemistry
Biosensors

Find what you need at Amazon.com

Lab supplies from Amazon