MIPs logo MIPdatabase      MIP2018 Conference website
Custom Search
Reference type: Journal
Authors: Gupta G, Bhaskar ASB, Tripathi BK, Pandey P, Boopathi M, Rao PVL, Singh B, Vijayaraghavan R
Article Title: Supersensitive detection of T-2 toxin by the in situ synthesized π-conjugated molecularly imprinted nanopatterns. An in situ investigation by surface plasmon resonance combined with electrochemistry.
Publication date: 2011
Journal: Biosensors and Bioelectronics
Volume: 26
Issue: (5)
Page numbers: 2534-2540.
DOI: 10.1016/j.bios.2010.10.050
Alternative URL: http://www.sciencedirect.com/science/article/B6TFC-51D15T8-3/2/3c4116bdf565adaa92f92031a5255c0e

Abstract: A π-conjugated molecularly imprinted polymer (MIP) with nanopatterns for T-2 toxin (T-2) was prepared on SPR chip by in situ electropolymerization of 3-aminophenylboronicacid (3-APBA) with T-2. The complete removal of T-2 from polymer was confirmed in situ by SPR and EIS and also ex situ by SEM, EDAX, fluorescence microscopy and Raman spectroscopy. SEM image of T-2 MIP exhibited nanopatterns due to imprinting of T-2. The MIP of T-2 showed a linear response for T-2 from 2.1 fM to 33.6 fM with a detection limit of 0.1 fM (0.05 pg/mL). In this study, thermodynamic parameters such as change in Gibb's free energy (Δ G), change in enthalpy (Δ H) and change in entropy (Δ S) were determined and the values revealed that the interaction between T-2 and T-2 MIP as spontaneous, endothermic and entropy driven one. Moreover, interactions of very high concentration of interferents with T-2 MIP showed very less response due to the presence of nanopatterns of T-2 in the T-2 MIP. Equilibrium constant (12.7 fM) obtained in this study indicates the super binding affinity of T-2 with T-2 MIP. Moreover, the present methodology provides an outline to develop field-detection equipment capable of detecting T-2 toxin at or well below the guideline concentrations recommended by American subcommittee on military field drinking water
Template and target information: T-2 toxin, T-2
Author keywords: π-Conjugated molecularly imprinted polymer, Biological warfare agent, surface plasmon resonance, Impedance, T-2 toxin, 3-APBA


  Mister Benzene Time for Tea mug  Chemistry peptide mug  Beach bunny periodic table shirt

Molecules Special Issue call






Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:
US listings   UK listings

Searching for Molecular Imprinting books on Amazon.com:
Molecular Imprinting
Imprinted Polymers
Polymer Chemistry
Organic Chemistry
Biosensors

Find what you need at Amazon.com

Lab supplies from Amazon