MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Huang BY, Chen YC, Wang GR, Liu CY
Article Title: Preparation and evaluation of a monolithic molecularly imprinted polymer for the chiral separation of neurotransmitters and their analogues by capillary electrochromatography.
Publication date: 2011
Journal: Journal of Chromatography A
Volume: 1218
Issue: (6)
Page numbers: 849-855.
DOI: 10.1016/j.chroma.2010.12.054
Alternative URL: http://www.sciencedirect.com/science/article/B6TG8-51S25HB-6/2/32943b05099f1f1c29c8a8807a480950

Abstract: A monolithic molecularly imprinted polymer (MIP) column was prepared as the stationary phase for the capillary electrochromatographic (CEC) separation of a group of structurally related compounds including dopamine (DA), ()-epinephrine (EP), (-)-isoproterenol (ISO), ()-norepinephrine (NE), ()-octopamine (OCT), and ()-synephrine (SYN). Here, (-)-NE was used as the template. Either methacrylic acid (MAA) or itaconic acid (IA) together with a mixture of ethylene glycol dimethacrylate (EDMA) and α,α'-azobis(isobutyronitrile) (AIBN) in N,N-dimethylformamide (DMF) was introduced into a pre-treated, silanised, fused-silica capillary by a thermal non-covalent polymerisation procedure. Optimised conditions for the polymerisation reaction were assessed by the separation efficiency of the template. Both the template/monomer/cross linker molar ratio and the compositions of the functional monomer, cross-linker, and porogen affected polymerisation. The optimum in situ polymerisation reaction was performed at 65 C for 17 min. By varying CEC parameters like eluent composition and pH, we observed that the addition of SDS to the eluent clearly improved the CEC separations. With a mobile phase of citrate buffer (10 mM, pH 3)/SDS (40 mM)/acetonitrile (2/2/1, v/v/v) solution and an applied voltage of 10 kV, the six related structures of the template and their enantiomeric mixtures were satisfactorily separated at 30 C
Template and target information: norepinephrine, NE, noradrenalin, dopamine, DA, epinephrine, EP, adrenalin, isoproterenol, ISO, octopamine, OCT, synephrine, SYN
Author keywords: capillary electrochromatography, chiral separation, molecularly imprinted polymer, monolith, Norepinephrine, template

  Periodic table Stew - the chemical formula for stew shirt  Periodic table Bag bag  Meerkat somebody mention coffee mug

Molecules Special Issue call      Appeal for information


Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.

Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:

Mickey Mouse 90th Anniversary banner