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Reference type: Journal
Authors: Huang BY, Chen YC, Wang GR, Liu CY
Article Title: Preparation and evaluation of a monolithic molecularly imprinted polymer for the chiral separation of neurotransmitters and their analogues by capillary electrochromatography.
Publication date: 2011
Journal: Journal of Chromatography A
Volume: 1218
Issue: (6)
Page numbers: 849-855.
DOI: 10.1016/j.chroma.2010.12.054
Alternative URL: http://www.sciencedirect.com/science/article/B6TG8-51S25HB-6/2/32943b05099f1f1c29c8a8807a480950

Abstract: A monolithic molecularly imprinted polymer (MIP) column was prepared as the stationary phase for the capillary electrochromatographic (CEC) separation of a group of structurally related compounds including dopamine (DA), ()-epinephrine (EP), (-)-isoproterenol (ISO), ()-norepinephrine (NE), ()-octopamine (OCT), and ()-synephrine (SYN). Here, (-)-NE was used as the template. Either methacrylic acid (MAA) or itaconic acid (IA) together with a mixture of ethylene glycol dimethacrylate (EDMA) and α,α'-azobis(isobutyronitrile) (AIBN) in N,N-dimethylformamide (DMF) was introduced into a pre-treated, silanised, fused-silica capillary by a thermal non-covalent polymerisation procedure. Optimised conditions for the polymerisation reaction were assessed by the separation efficiency of the template. Both the template/monomer/cross linker molar ratio and the compositions of the functional monomer, cross-linker, and porogen affected polymerisation. The optimum in situ polymerisation reaction was performed at 65 C for 17 min. By varying CEC parameters like eluent composition and pH, we observed that the addition of SDS to the eluent clearly improved the CEC separations. With a mobile phase of citrate buffer (10 mM, pH 3)/SDS (40 mM)/acetonitrile (2/2/1, v/v/v) solution and an applied voltage of 10 kV, the six related structures of the template and their enantiomeric mixtures were satisfactorily separated at 30 C
Template and target information: norepinephrine, NE, noradrenalin, dopamine, DA, epinephrine, EP, adrenalin, isoproterenol, ISO, octopamine, OCT, synephrine, SYN
Author keywords: capillary electrochromatography, chiral separation, molecularly imprinted polymer, monolith, Norepinephrine, template


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