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Reference type: Journal
Authors: Prasad BB, Tiwari MP, Madhuri R, Sharma PS
Article Title: Enantioselective separation and electrochemical sensing of d- and l-tryptophan at ultratrace level using molecularly imprinted micro-solid phase extraction fiber coupled with complementary molecularly imprinted polymer-fiber sensor.
Publication date: 2011
Journal: Journal of Chromatography B
Volume: 879
Issue: (5-6)
Page numbers: 364-370.
DOI: 10.1016/j.jchromb.2010.12.023
Alternative URL: http://www.sciencedirect.com/science/article/B6X0P-51V5GN5-3/2/dac0a95aaec35462f76340cf586aa7b7

Abstract: Highly efficient enantioselective separation and quantitative recoveries of d- and l-tryptophan in aqueous and real samples can be achieved, with a monolithic molecularly imprinted polymeric fiber that serves both for micro-solid phase extraction and ultratrace sensing, without any false-positive (non-specific) contribution and cross-reactivity, in the range of 0.15-30.00 ng mL-1 with detection limit as low as 0.0261 ng mL-1 (relative standard deviation = 0.64%, signal/noise = 3). The proposed method combining molecularly imprinted micro-solid phase extraction fiber and a complementary molecularly imprinted polymer-carbon composite fiber sensor is proven to be useful for clinical diagnosis of stress-related diseases caused by acute tryptophan depletion
Template and target information: tryptophan, l-tryptophan, d-tryptophan
Author keywords: Enantioseperation, Molecularly imprinted micro-solid phase extraction fiber, l- and d-Tryptophan, Differential pulse anodic stripping voltammetry


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