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Abstract: The need for chemical and biological entities of predetermined selectivity and affinity towards target analytes is greater than ever, in applications such as environmental monitoring, bioterrorism detection and analysis of natural toxin contaminants in the food chain. In this review, we focus on advances in the production of specific binders, in terms of both natural entities (e.g., antibodies) and synthetic binders (e.g., molecularly-imprinted polymers). We discuss the potential of emerging technologies for integration into immunoassay and sensing techniques. We place special emphasis on use of these technologies in bioanalytical applications
Template and target information: Review - sensors and assays for small molecules
Author keywords: antibody, aptamer, Binding protein, biorecognition, immunoassay, molecular-imprinted polymer, Nanobody, Protein scaffold, Single-chain variable fragment, Synthetic binder