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Reference type: Journal
Authors: Helling S, Shinde S, Brosseron F, Schnabel A, Müller T, Meyer HE, Marcus K, Sellergren B
Article Title: Ultratrace Enrichment of Tyrosine Phosphorylated Peptides on an Imprinted Polymer.
Publication date: 2011
Journal: Analytical Chemistry
Volume: 83
Issue: (5)
Page numbers: 1862-1865.
DOI: 10.1021/ac103086v

Abstract: Novel molecularly imprinted polymers (MIPs) designed to bind the side chain of phosphotyrosine can be used as artificial receptors for affinity-based enrichment of proteolytic peptides. In comparison with general enrichment methods for phosphorylated peptides such as TiO2-based methods, the pTyr-imprinted polymers offered high selectivity for pTyr-containing peptides down to the low fmol level. This suggests MIPs as a new tool for affinity-based proteomics
Template and target information: phosphorylated peptides, phospho-tyrosine, pTyr


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