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Reference type: Journal
Authors: Lin JM, Nakagama T, Uchiyama K, Hobo T
Article Title: Capillary electrochromatographic separation of amino acid enantiomers using on-column prepared molecularly imprinted polymer.
Publication date: 1997
Journal: Journal of Pharmaceutical and Biomedical Analysis
Volume: 15
Issue: (9-10)
Page numbers: 1351-1358.
DOI: 10.1016/S0731-7085(96)02013-4

Abstract: The use of molecularly imprinted polymer polymerized in capillary for the separation of amino acid enantiomers by electrochromatography is described. The substrate-selective polymers were prepared by using L- phenylalanine anilide as print molecule and methacrylic acid and/or 2-vinylpyridine as the functional monomers, which is believed to interact both ionically and through hydrogen bonding with the print molecule. Several aspects of the polymer preparation were investigated, including the treatment of the inside surface of the capillary, the composition of the polymers and the running conditions of the capillary electrochromatography. Such separation was highly specific and depended on the presence of both the print molecule and the functional monomer in the polymerization mixture. This preliminary report demonstrates a novel and simple method for the development of the capillary electrochromatographic separation of amino acid enantiomers using molecularly imprinted polymer. (C) 1997 Elsevier Science B.V


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