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Abstract: Objecive A method for extraction of amphetamines in blood samples using molecularly imprinted solid phase extraction and gas chromatography -mass spectrometry (GC/MS). Methods Human blood samples were spiked with amphetamine, methamphetamine, MDA and MDMA, and diluted with 4:1 (v/v) with 10mmol/L ammonium acetate buffer (pH8.0); activating the MIP column with 1mL methanol and 1mL 10mmol/L ammonium acetate(pH8.0); Washing the impurities using 21mL water, 1mL 60/40 MeCN/water and 1mL 1% HAc in MeCN; Elute the amphetamine drugs with 21mL 1% formic acid in methanol, then evaporate under nitrogen to dryness and reconstitute with 100 μL methanol prior to GC/MS analysis. Results The recoveries obtained with this method for amphetamine drugs are more than 90%. The linear range was 20 ~ 5000ng/mL with correlation coefficients between 0.995 7 and 0.998 9. The limits of detection were 20ng/mL for amphetamine (AM), 16ng/mL methamphetamine (MA), 30ng/mL methylenedioxyamphetamine (MDA) and methylened ioxy methamp hetamine (MDMA), The limits of quantitative were 10ng/mL for AM, 8ng/mL for methamphetamine (MAM), 15ng/mL for MDA and MDMA. Conclusion Recoveries are higher, impurities were less. The method could be applied for simultaneous determination of trace amphetamines in biological specimens
Template and target information: amphetamine, AM, methamphetamine, MAM, methylenedioxyamphetamine, MDA, MDMA, methylenedioxymethamphetamine
Author keywords: Amphetamines, Forensic toxicology analysis, GC, MS, GC, NPD, molecularly imprinted solid phase extraction