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Reference type: Journal
Authors: Gai QQ, Qu F, Zhang T, Zhang YK
Article Title: The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation.
Publication date: 2011
Journal: Journal of Chromatography A
Volume: 1218
Issue: (22)
Page numbers: 3489-3495.
DOI: 10.1016/j.chroma.2011.03.069
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0021967311004444

Abstract: Currently, small proteins imprinting are more reported since large proteins molecular imprinting faces challenge due to their bulk size and complex structure. In this work, bovine serum albumin (BSA) surface-imprinted magnetic polymer was successfully synthesized based on atomic transfer radical polymerization (ATRP) method in the presence of common monomer (N-isopropylacrylamide) with the assistant of basic functional monomer (N-[3-(dimethylamino)propyl]-methacrylamide), which provides a achievable attempt for imprinting larger target proteins based on the ATPR with the mild reaction conditions. The BSA-imprinted polymer exhibited higher adsorption capacity and selectivity to BSA over the non-imprinted polymer. Competitive adsorption tests indicated the BSA-imprinted polymer had better selective adsorption and recognition properties to BSA in the mixture. The obtained BSA-imprinted polymer was applied to bovine serum, which also showed selectivity to BSA. In addition, a conventional aqueous two-phase solution of PEG/sulphate was used as elution for adsorbed BSA, which was compared with common NaCl elution
Template and target information: protein, bovine serum albumin, BSA
Author keywords: molecularly imprinted polymer, magnetic particles, Bovine serum albumin, Protein separation


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