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Reference type: Journal
Authors: Liu XC, Mosbach K
Article Title: Catalysis of benzisoxazole isomerization by molecularly imprinted polymers.
Publication date: 1998
Journal: Macromolecular Rapid Communications
Volume: 19
Issue: (12)
Page numbers: 671-674.
DOI: 10.1002/(SICI)1521-3927(19981201)19:12<671::AID-MARC671>3.0.CO;2-6

Abstract: A tailor-made catalytically active polymer catalyzing the benzisoxazole isomerization is described. Kinetic studies carried out in water/ethanol (3:1, v/v) at room temperature, showed a rate acceleration (k(MIP)/k(control)) of 7.2-fold compared to the control polymer. The imprinted polymer exhibits Michaelis-Menten kinetics with a K-m of 0.484 mM and a k(cat) of 0.205 min(-1). Compared with the uncatalyzed reaction, a rate enhancement ((k(cat)/K-m)/k(uncat)) of 4 x 10(4) fold was obtained. Substrate selectivity, accessible binding site analysis, dissociation constant determination, and inhibition study were also performed

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