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Abstract: A simple and very sensitive capillary-liquid chromatography method coupled with laser induced fluorescence detection has been developed for the simultaneous determination of seven quinolones of veterinary use in milk. Moreover, a comparison between two different sample treatments (QuEChERS and molecularly imprinted polymer, MIP) has been carried out in terms of efficiency of the extraction (number of analytes to be analysed and absence of interferences), throughput, linear dynamic range in matrix-matches calibrations, detection and quantification limits and accuracy (trueness and precision, by means of recovery assays). The results showed that the QuEChERS procedure was more efficient and faster, showing good recoveries, sensitivity and precision for all the studied compounds. Employing this proposed method, very low detection limits, between 0.4 μg/kg for danofloxacin, and 6 μg/kg for sarafloxacin, have been obtained
Template and target information: danofloxacin, DANO, sarafloxacin, SARA, enrofloxacin, ENRO, ciprofloxacin, CIPRO, difloxacin, DIFLO, oxolinic acid, OXO, flumequine, FLUME
Author keywords: Quinolones, Capillary HPLC, Laser induced fluorescence detection, QuEChERS, Molecularly imprinted polymers