Proceedings title: Advanced Materials Research
Editors: Zhang H, Jin D
Publisher: Trans Tech Publications
Volume number: 282-283
Conference information: Advanced Research on Material Engineering, Chemistry, Bioinformatics
Abstract: Objective: To prepare a novel protein molecularly imprinted membrane. Methods: Konjac glucomanan was swollen, cross-linked, added papain and glycerol, dried to form imprinted membranes. Results: After eluted, the rebinding adsorption amounts of papain for imprinted and blank membranes were (12.692 ± 3.452) μg/cm2 and (7.849 ± 2.321) μg/cm2 respectively. The papain imprinted membrane had the highest imprinted factor a 1.8 for papain than for other proteins. Results of FT-IR and SEM showed that the cavity structure of the eluted membrane provided space for rebinding. Conclusions: Konjac glucomannan-based imprinted membrane has good specific adsorption and selective recognition abilities for the templates- papain, and shows great possibilities to be a promising protein molecularly imprinted polymer.
Template and target information: protein, papain
Author keywords: Konjac glucomannan, molecularly imprinted membrane, papain