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Reference type: Conference Proceeding
Authors: Mi ZY, Ma Z, Li XL, Xiong WD, Zhang YQ
Publication date: 2011
Article title: Recognition of Papain by Konjac Glucomannan-Based Molecularly Imprinted Membrane.
Page numbers: 687-690
DOI: 10.4028/www.scientific.net/AMR.282-283.687 Alternative URL: http://www.scientific.net/AMR.282-283.687

Proceedings title: Advanced Materials Research
Editors: Zhang H, Jin D
Publisher: Trans Tech Publications
Volume number: 282-283
Conference information: Advanced Research on Material Engineering, Chemistry, Bioinformatics

Abstract: Objective: To prepare a novel protein molecularly imprinted membrane. Methods: Konjac glucomanan was swollen, cross-linked, added papain and glycerol, dried to form imprinted membranes. Results: After eluted, the rebinding adsorption amounts of papain for imprinted and blank membranes were (12.692 3.452) μg/cm2 and (7.849 2.321) μg/cm2 respectively. The papain imprinted membrane had the highest imprinted factor a 1.8 for papain than for other proteins. Results of FT-IR and SEM showed that the cavity structure of the eluted membrane provided space for rebinding. Conclusions: Konjac glucomannan-based imprinted membrane has good specific adsorption and selective recognition abilities for the templates- papain, and shows great possibilities to be a promising protein molecularly imprinted polymer.
Template and target information: protein, papain
Author keywords: Konjac glucomannan, molecularly imprinted membrane, papain


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