Abstract: A group selective molecularly imprinted solid phase extraction(MISPE) for malachite green(MG) from fish water and fish feed samples was developed.Using MG as template molecule,methacrylic acid as functional monomer,ethylene glycoldimethacrylate as linking agent and bulk polymerization as synthetic method,the molecularly imprinted polymers(MIPs) were synthesized and characterized with rebinding experiment.The extraction conditions of MISPE column for MG were optimized.For real samples,no detectable MG was observed in one fish farming water sample and two fish feed samples;while the MG concentrations in two pet fishpond water samples were found at 1.50 ngˇmL-1 and 0.67 ngˇmL-1,respectively.
Template and target information: malachite green, MG
Author keywords: Molecularly imprinted polymers, Malachite green, solid phase extraction, high performance liquid chromatography