Volume number: Analytical Chemistry
Conference information: Proceedings of PACCON2011 (Pure and Applied Chemistry International Conference 2011)
Abstract: Molecularly imprinted polymers (MIPs) for selectively detecting allergen proteins from natural rubber latex were used to prepare a sensor material for a quartz crystal microbalance (QCM). Recognition sites are created for Hevein latex allergen proteins by varying the polymer components with stamp imprinting procedures using Hev b1 obtained from rubber gloves as the template. The selective recognition by the sensor layer of Hev b1 allergen proteins was obtained with copolymers yielded from methacrylic acid-vinylpyrrolidone-dihydroxyethylenebisacrylamide cross-linked polymers. The imprinted materials can be applied to distinguish latex allergen Hev b1 from such analogues as lysozyme, ovalbumin and bovine serum albumin. The sensor layers allow selective Hev b1 adsorption within minutes in amounts ranging from 10 to 1000 μg l-1 and with a detection limit of 1 μg l-1. The imprinted cavities recognize their receptor binding sites and distinguish related hevein latex allergenic proteins isolated from natural rubber latex (Hev b1, Hev b2 and Hev b3) with a selectivity factor of 4 to 7. The sensor provides a fast and reliable response to natural rubber latex protein, even in real matrices obtained by extracting rubber gloves.
Template and target information: protein, Hevein latex allergen proteins, latex allergen, Hev b1