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Volume number: Analytical Chemistry
Conference information: Proceedings of PACCON2011 (Pure and Applied Chemistry International Conference 2011)

Abstract: An interdigitated capacitive immunosensor for detection of rubber tree latex allergens in rubber gloves was developed by using molecularly imprinted polymer (MIP) on an interdigitated capacitance (IDC) microelectrode. Based on the change in the dielectric constant of an IDC and the MIP film consisting of the biomimetic recognition polymer material transduces binding events of specific protein-MIP binding upon the analyte binds, was introduced for the measurement of the capacitance of the IDC microelectrode system. The functionalized layers were prepared from a crosslinked poly(methacrylic acid-co-vinylpyrrolidone-co-dihydroxy ethylene bisacrylamide) and the binding of the latex allergen Hev b1 (rubber elongation factor). They recognized Hevea latex proteins (Hev b1, Hev b2, or Hev b3) better (by a factor of 2) than non-Hev b proteins (lysozyme, ovalbumin and bovine serum albumin) which can be distinguished by the imprinted layers, depending on the dimension and conformation of these proteins (selectivity factor values of at least 4). The sensitivity (range 10-900 ng ml-1) and selectivity achieved with Hev b1 latex allergens in pH 7.4 phosphate buffer was good with the detection limit of 10 ng ml-1. The total response time of sensor is in the order of 3 min. The present work has demonstrated that the proposed assay can be a fast, reliable and inexpensive detection method for the major latex allergen protein Hev b1 in a complex matrix obtained by extraction from rubber gloves.
Template and target information: protein, Hevein latex allergen proteins, latex allergen, Hev b1, Hev b2, Hev b3