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Conference information: Abstracts, 15th Iranian Chemistry Congress

Abstract: sample cleanup technique combined with high performance liquid chromatography In Sodium in biological fluids using molecularly imprinted solid-phase extraction as the this work, a novel method is described for the determination of Cefuroxime (HPLC). The molecularly imprinted polymers (MIPs) were prepared using methacrylic acid and Allyl glycidil ether/iminodiacetic acid as the functional monomer and N, N-methylene diacrylamid as the cross-linker in methanol solution using cefuroxime sodium as the template molecule and 2,2-azobis-isobutyronitrile as the initiator. The novel imprinted polymer was used as a solid-phase extraction sorbent for the extraction of cefuroxime sodium from human serum and urine. Various parameters affecting the extraction efficiency of the polymer have been evaluated. The optimal conditions for molecularly imprinted solid-phase extraction (MISPE) consisted of conditioning 1mL methanol and 1mL of deionizedwater at neutral pH, loading of 5mL of the water sample (25_gL -1) at pH 4.0, washing using 2mL acetonitrile/acetone (1/4, v/v) and elution with 3× 1mL methanol/acetic acid (10/0.01, v/v). The MIP selectivity was evaluated by checking several substances with similar molecular structures to that of cefuroxime sodium. Results from the HPLC analyses showed that the calibration curve of cefuroxime sodium using MIP from human serum and urine is linear in the ranges of 0.5-100 and 1.5–100_gL -1 with good precisions (3.7% and 3.1% for 5.0_gL -1), respectively. The recoveries for serum and urine samples were higher than 92%
Template and target information: Cefuroxime Sodium