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Abstract: Molecularly imprinted polymeric microspheres(MIPMs) were prepared by one-step swelling polymerization method in aqueous system using micron-polystyrene microspheres prepared by emulsifer-free polymerization as seeds and using N-methylasparagic acid(NMA) as cyclostyle molecules.Good monodispersed MIPMs were prepared with particle size in the range of 2~3 µm.Scatchard analysis suggested the MIPMs recognizing NMA with two classes of binding sites.The calculated dissociation constant KD1 and apparent maximum number Bmax1 of binding sites with high affinity were 0.223 mmol/L and 2.12 μmol/g respectively,while KD2 and Bmax2 of binding sites with low affinity were 5.34 mmol/L and 36.42 μmol/g.
Template and target information: N-methylasparagic acid, NMA
Author keywords: molecularly imprinted polymeric microspheres, Single-step swelling polymerization, monodispersity, binding sites