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Abstract: Objective To study the efficacy of using cholesterol molecular imprinted polymers(MIPs) as solid-phase extraction(SPE) sorbent in pretreatment of different biological samples.Methods MIPs for cholesterol were prepared using methacrylic acid(MAA) as a monomer and ethylene glycol dimethacrylate(EDMA) as a cross-linker.Both thermal and radical polymerization was studied.First the binding characters of 2 MIPs studied.Then the MIPs were used as SPE sorbents for selectively trapping and pre-concentration of cholesterol from human serum,milk and yolk.The SPE sorbent was compared with C18 SPE sorbent.Results Cholesterol MIPs could recognize and selectively bind to cholesterol.The binding capability and selectivity of radical polymerization MIPs were better than that of thermal polymerization.Washing and elution of the cholesterol were critical in MISPE.The optical extraction protocol was: conditioning MISPE cartridges with n-hexane,loading with n-hexane,washing the cartridges with n-hexane and n-hexane:toluene=9:1 respectively,then eluting with chloroform:ethanol:acetic acid=3:1:1.The recovery was over 70%,better than that of C18 SPE(recovery).Conclusion Cholesterol MIPs can selectively recognize and separate cholesterol,and can be successfully applied to the extraction of cholesterol from biological samples.
Template and target information: cholesterol
Author keywords: cholesterol, molecular imprinted polymers, solid-phased extraction, biological samples