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Abstract: Molecularly Imprinted polymers are the state-of-the art in production of man-made 'smart' materials that selectively recognize a target molecule, the template. They are produced by the 'frozen' of monomers around the template, which are initially pre-organized into a solution, by polymerization. After template removal the remaining into polymer cavity are both in shape and chemical functionality complementary to the template, so selectively could recognize it. The monomers methacrylic acid (MAA, functional monomer) and ethylenoglycol dimethacrylate (EDMA, cross-linker) are used extensively in such process, considering as the most appropriate. In present study were used as template the peptide the Arg-Gly-Asp with two common functional monomers, MAA and acrylamide, and as cross-linker the EDMA. As it is already well known the RGD peptide is the major integrin binding site present in a variety of integrin ligands. The molar ratio of template-functional monomer-cross-linker was kept constant for both monomers and their effect in recognition properties of produced polymers was reported. The RGD rebinding results are indicating that in case of MAA the produced polymers have smaller dissociation constant KD values in a Langmuir monosite model isotherm meaning better recognition, but the values of the percentage of theoretical maximum binding sites (% Bmax) are lower than the case of acrylamide. These results suggest that the acrylamide, despite the lower somewhat KD, are the monomer of choice for the production of such polymers for RGD recognition leading to higher imprinting efficiency.
Template and target information: Arg-Gly-Asp