Abstract: A molecularly-imprinted polymer(MIP) was prepared by bulk polymerization, using kaempferol as the template molecule, 4-vinyl pyridine as the functional monomer, and ethylene glycol dimethacrylate as the cross-linker in the solvent system of CHCl3-DMF(3:1, volume ratio). The synthesized polymer, i.e.kaempferol-MIP, was analyzed by FT-IR and scanning electron microscopey(SEM). In addition, the adsorption properties and mechanism were studied as well. The experimental results showed that the kaempferol-MIP exhibited a favorable affinity for kaempferol. The Scatchard analysis reveals that there exists a class of homogenous recognition sites in kaempferol-MIP within the range of certain concentration. Apparent maximal combination amount (Qmax) and equilibrium dissociation constant (KD) were calculated to be 3 938 μg/g and 9.074 mg/L, respectively. The result of selectivity study showed that the selectivity coefficient is 3.24 with respect to kaempferol-rutin system.
Template and target information: kaempferol
Author keywords: molecularly-imprinted polymer, kaempferol, Specific recognition, Adsorption properties