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Abstract: As a new technique of recent development, molecular imprinting technique(MIT) with predetermination, specific recognition and practicability is widely used in the domain of environment, biological medicines, and so on .It also has important research potential in food security test. In this paper, using chlorpyrifos as template, the molecular imprinted polymers was prepared by in situ step-addition polymerization. The characteristics of selective separation and pre-concentration for chlorpyrifos, dichlorovos and methamidophos were examined on the polymer. The chlorpyrifos from the honey,vegetables and natural water was extracted via using the polymer. The extraction conditions of MISPE column for chlorpyrifos were optimized. The results indicated that different kinds of recognition sites in the MIPs showed specific affinity toward chlorpyrifos. Velocity is 1.0 mL.min -1, the sampling volume of 20 μL Average recoveries for chlorpyrifos in a blank sample spiked at 3 levels were between 96.0% and 102%, with a RSD of less than 6.6%. This method is easily operated, highly selective and has no requirement of complicated sample pretreatment and is applicable for the determination of chlorpyrifos residue in foods.
Template and target information: chlorpyrifos
Author keywords: Molecularly imprinted polymers, Chlorpyrifos, high-performance liquid chromatography