MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Bereli N, Saylan Y, Uzun L, Say RI, Denizli A
Article Title: l-Histidine imprinted supermacroporous cryogels for protein recognition.
Publication date: 2011
Journal: Separation and Purification Technology
Volume: 82
Issue: (1)
Page numbers: 28-35.
DOI: 10.1016/j.seppur.2011.08.011
Alternative URL: http://www.sciencedirect.com/science/article/pii/S1383586611004710

Abstract: Molecular imprinting is a method for making selective binding sites in synthetic polymers using a molecular template. The aim of this study is to prepare l-histidine-imprinted poly(hydroxy ethylmethacrylate) based supermacroporous cryogels which can be used for the purification of lysozyme from egg white. A metal chelate monomer [N-methacryloyl-(l)-histidinemethylester (MAH)] forming coordination complex with the template l-histidine in the presence of Cu2+ ions copolymerized with hydroxyethyl methacrylate (HEMA), using N,N'-methylene-bis(acrylamide) (MBAAm) as the cross-linker and ammonium persulfate (APS)/N,N,N',N'-tetramethylene diamine (TEMED) as initiator/activator pair to prepare the MIP cryogel. After that, the template (i.e., l-histidine) was removed using 1 M KSCN solution. The maximum lysozyme adsorption amount was 54.2 mg/g polymer. The relative selectivity coefficients of the MIP cryogel for lysozyme/ribonuclease A and lysozyme/cytochrome c were 4.5 and 2.4 times greater than the non-imprinted poly(HEMA-MAH) (NIP) cryogel, respectively. The resulting MIP cryogels possess excellent long term storage stability and could be used many times without decreasing the adsorption amount significantly
Template and target information: l-histidine, protein, lysozyme
Author keywords: Cryogels, molecular imprinting, molecular recognition, Lysozyme purification, affinity binding


  SMI logo tie  Mug featuring the name Adil spelled out in the single letter amino acid code    Shirt featuring the template Lysozyme

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner