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Reference type: Conference Proceeding
Authors: Mi ZY, Ma Z, Li XL, Wang P, Zhang YQ
Publication date: 2012
Article title: Preparation and Characterize of Konjac Glucomannan-Based Protein Molecularly Imprinted Polymer.
Page numbers: 460-463
DOI: 10.4028/www.scientific.net/AMR.366.460 Alternative URL: http://www.scientific.net/AMR.366.460

Proceedings title: Advanced Materials Research
Editors: Zhang H, Jin D
Publisher: Trans Tech Publications
City: Switzerland
Volume number: 366
Conference information: Advanced Research on Material Engineering, Architectural Engineering and Informatization

Abstract: Objective: To prepare a novel molecularly imprinted materials for protein recognition. Methods: Konjac glucomannan was used as fundamental materials, after swollen, added 3-Chloro-1,2- epoxypropane and glycerol cross-linked for 3 h at 40 C. Then added bovine serum albumin (BSA) solution, agitated 1 h at room temperature. Natural dried. Results: After eluted, the imprinted efficiency of the imprinted membrane were (59.273.53) % (n=6) with blank membrane as control. The adsorption rate increased from 2 h to 20 h, and reached saturation at 22 to 24 h. The selectively recognition experiment indicated the BSA imprinted membrane have significant selectively recognition abilities for the template protein. Conclusions: konjac glucomannan-based imprinted membrane shows great possibilities to be a promising material for protein molecularly imprinted recognition.
Template and target information: protein, BSA, bovine serum albumin
Author keywords: Bovine serum albumin, Konjac glucomannan, Protein Molecularly Imprinted Polymer


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