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Abstract: A novel molecular imprinted solid-phase extraction technique was developed by using microspheres prepared by modifying the silica through a sol-gel process with a layer of homogeneous and compact molecularly imprinted polymer (MIP), which was synthesized with 3-aminopropyltriethoxysilane and phenyltrimethoxysilane as functional monomers, diethylstilbestrol (DES) as the template, and tetraethoxysilane as the cross-linker. The saturated adsorption capacity of the MIP under the condition of continuous loading of target solution was 0.213 mg g-1, and the imprinting factor reached 6.0. The selectivity factor of the MIPs for bisphenol A and 4-tert-butylphenol were 3.5 and 9.6, respectively. The molecular imprinted solid-phase extraction (MISPE) is a viable and efficient technique for extraction of DES from chicken samples with a recovery ofmore than 85%. The limit of detection (LOD) was determined to be 8 ng g-1.
Template and target information: diethylstilbestrol, DES
Author keywords: diethylstilbestrol, molecularly imprinted polymer, sol-gel, solid-phase extraction microsphere