MIPs logo MIPdatabase      MIP2024 Conference banner, website is now open, register on site for important updates   
Custom Search
Reference type: Journal
Authors: Sener G, Uzun L, Say RI, Denizli A
Article Title: Use of molecular imprinted nanoparticles as biorecognition element on surface plasmon resonance sensor.
Publication date: 2011
Journal: Sensors and Actuators B: Chemical
Volume: 160
Issue: (1)
Page numbers: 791-799.
DOI: 10.1016/j.snb.2011.08.064
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0925400511007878

Abstract: In this study, we have prepared lysozyme imprinted poly(ethylene glycol dimethacrylate-N-methacryloyl-l-histidine methylester) (PEDMAH) nanoparticles and then attached on the surface of surface plasmon resonance (SPR) sensor. Lysozyme imprinted SPR nanosensor was characterized by Fourier transform infrared spectroscopy, atomic force microscopy, and ellipsometry. The nanosensor has an ability to detect lysozyme molecules from both aqueous and natural complex source, chicken egg white, even if it has lysozyme concentration as low as 32.2 nM. Association kinetics analysis, Scatchard, Langmuir, Freundlich, Langmuir - Freundlich isotherms were applied to data. The calculated detection limit, association and dissociation constants are 84 pM, 108.71 nM-1 and 9.20 pM, respectively. The non-imprinted nanosensors were also prepared to evaluate the selectivity of the imprinted nanosensor. Finally, the nanosensor is used for five adsorption - desorption - regeneration cycle and it gives reproducible response
Template and target information: protein, lysozyme
Author keywords: lysozyme, molecular imprinting, nanoparticles, Surface plasmon resonance nanosensor, Chicken egg-white


  Molybdenum periodic table mug  Mug featuring the name Adil spelled out in the single letter amino acid code    Shirt featuring the template Lysozyme






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner