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Reference type: Journal
Authors: Zhang QJ, Su YJ, He QQ, Shen XG, He LM, Zhang N, Zeng ZL
Article Title: Molecularly imprinted solid-phase extraction for the selective HPLC determination of ractopamine in pig urine.
Publication date: 2011
Journal: Journal of Separation Science
Volume: 34
Issue: (23)
Page numbers: 3399-3409.
DOI: 10.1002/jssc.201100655

Abstract: A method was developed for the determination of ractopamine in pig urine using molecularly imprinted solid-phase extraction (MISPE) as the sample clean-up technique combined with high-performance liquid chromatography. The molecularly imprinted polymer (MIP) was synthesized in acetonitrile-triethylamine system using ractopamine (RAC) as the template and acrylamide as the monomer. The binding capacity of the polymer toward RAC was found to be about 2.57 mg of ractopamine/g of polymer. The optimal procedures for MISPE consisted of conditioning with 3 mL methanol, equilibrating with 3 mL of water, loading volume of <10 mL of aqueous sample (pH 7), washing with 3 mL water and 3 mL methanol, and eluting with 5 mL of 5% ammonia in methanol. In the four spiked samples with the levels of 0.01, 0.1, 1.0 and 5.0 μg/mL, the mean recoveries of analyte on the MIP were higher than 90% with relative standard deviation <10%, and significant differences between imprinted and non-imprinted materials were observed. The MIP selectivity was evaluated by checking 11 drugs with similar and different molecular structures to that of RAC. The characteristics of three-dimensional cavities and hydrogen bond interaction were regarded as the main factors that dominated the retention of RAC on the MISPE cartridge
Template and target information: ractopamine, RAC
Author keywords: HPLC, Molecularly imprinted polymers, Pig urine, Ractopamine, SPE


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