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Abstract: Molecularly imprinted polymer (MIP) was prepared using acrylamide (AM) and ethylene glycol dimethacrylate (EDMA) as functional monomer and crosslinking monomer, respectively. Loureirin B [1-(4-hydroxyphenyl)-3-(2,4,6-trimethoxyphenyl)propan-1-one] was chosen as the template molecule and acetonitrile as the porogen. The recognition and binding characteristics of the MIP were evaluated by static equilibrium experiment. The Scatchard analysis showed that the dissociation constant and the apparent maximum number of the binding sites were 0.415 mmol/L and 276.48 μmol/g, respectively. The infrared spectrometry of the MIP indicated that there were some functional groups in the molecularly imprinted polymer which could interact with the template. Several washing and elution solvents were tested in the MIP extraction of real samples. It was observed that methanol was suitable for washing out of interferences and the best elution solvent was methanol with 10% acetic acid. The results revealed that the MIP possessed high selectivity for Loureirin B and good affinity to its structural analogues.
Template and target information: Loureirin B, 1-(4-hydroxyphenyl)-3-(2,4,6-trimethoxyphenyl)propan-1-one
Author keywords: binding characteristic, Dragon's Blood, Loureirin B, molecularly imprinted polymer, selective extraction