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Reference type: Conference Proceeding
Authors: Qiao FX, Yan HY
Publication date: 2011
Article title: Validation of the Pseudo Imprinted Microspheres for Solid-Phase Extraction of Acyclovir from Swine Tissue.
Page numbers: 1591-1594
DOI: 10.4028/www.scientific.net/AMR.393-395.1591 Alternative URL: http://www.scientific.net/AMR.393-395.1591

Proceedings title: Advanced Materials Research
Editors: Chen R, Sung WP
Publisher: Scientific.net
Volume number: 393-395
Conference information: Biotechnology, Chemical and Materials Engineering

Abstract: The novel pseudo imprinted microspheres were synthesized by aqueous suspension polymerization using theophylline as the template and employed as a special sorbent of solid-phase extraction for selective extraction of acyclovir from swine tissue. The pseudo imprinted microspheres revealed high affinity and selectivity to acyclovir, and the matrix interferences could be efficiently eliminated, which make the extract of solid-phase extraction clean enough for HPLC analysis. The parameters that affect the extraction efficiency of solid-phase extraction were investigated, including solvent of extraction, volume of loading sample, washing and elution solvent, etc. Good linearity was obtained in a range of 0.05~15 μg/mL with correlation coefficient (r2) of 0.9991. The recoveries at three spiked levels were ranged from 91.4% to101.0% with RSD less than of 5.0%.
Template and target information: acyclovir
Author keywords: Acyclovir, Pseudo Imprinted Polymer, Solid-Phase Extraction (SPE), Swine Tissue


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