MIPs logo MIPdatabase      Use this space
Custom Search
Reference type: Journal
Authors: Vidal JC, Duato P, Bonel L, Castillo JR
Article Title: Molecularly Imprinted On-Line Solid-Phase Extraction Coupled with Fluorescence Detection for the Determination of Ochratoxin A in Wheat Samples.
Publication date: 2012
Journal: Analytical Letters
Volume: 45
Issue: (1)
Page numbers: 51-62.
DOI: 10.1080/00032719.2011.565449

Abstract: An automatic solid-phase extraction (SPE) system coupled with a fluorescence detector was developed for the sensitive determination of Ochratoxin A (OTA) in wheat at the ng mL-1 levels. Cross-linked molecularly imprinted polymers (MIPs) were synthesized as sorbents by a non-covalent approach using N-(4-chloro-1-hydroxy-2-naphthoylamido)-(L)-phenylalanine (OTAm) as a mimic template, methacrylic acid as functional monomer, and ethylendimethacrylate as the cross-linker in chloroform porogenic solvent, polymerizing the mixture by thermal treatment at 60 C. These MIP polymers showed excellent affinity and specificity in the recognition of natural OTA mycotoxin compared to the results obtained with nonimprinted polymers. The polymers were slurry packed into a SPE column (V ~ 1.7 mL) and used in a SPE flowing system coupled to on-line molecular fluorescence detection (named MISPE-FLD) for the OTA determination. Methanol (MeOH), MeOH:H2O (1:9) + 1% acetic acid (AcOH), and MeOH:tributylamine (99:1) were tested as desorbing solvents. A dynamic binding capacity of 118 9 ng of OTA (n = 3) was calculated for 45 mg of dry MIP particles. The described instrument demonstrated the cleanup of the matrix by the imprinted column and the simultaneous determination of OTA in wheat samples in the range 3 - 18 ng mL-1 (LOD 1.2 ng mL-1 of OTA). The recoveries of reference wheat flour materials spiked with OTA were about 93 9%. The MISPE-FLD instrument can be used for concentrations below the maximum levels of the EC regulatory limits for OTA in unprocessed cereals (5.0 μg kg-1) (EC Commission Regulation 2006)
Template and target information: N-(4-chloro-1-hydroxy-2-naphthoylamido)-(L)-phenylalanine, OTAm, mimic template, ochratoxin A, OTA
Author keywords: fluorescence detection, molecularly imprinted polymers (MIPs), Ochratoxin A (OTA), Solid-Phase Extraction (SPE), Wheat samples


  Periodic table Banana Split - the chemical formula for banana split shirt  Periodic table Bag bag  Meerkat somebody mention coffee mug

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner