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Reference type: Journal
Authors: Qiu HM, Xi YL, Lu FG, Fan LL, Luo CN
Article Title: Determination of l-phenylalanine on-line based on molecularly imprinted polymeric microspheres and flow injection chemiluminescence.
Publication date: 2012
Journal: Spectrochimica Acta Part A-Molecular and Biomolecular Spctroscopy
Volume: 86
Issue: (1)
Page numbers: 456-460.
DOI: 10.1016/j.saa.2011.10.068
Alternative URL: http://www.sciencedirect.com/science/article/pii/S1386142511009826

Abstract: A novel molecular imprinting-chemiluminescence (MIP-CL) sensor for the determination of l-phenylalanine (Phe) using molecularly imprinted polymer (MIP) as recognition element is reported. The Phe-MIP was synthesized using acrylamide (AM) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker, 2,2-azobisisobutyronitrile (AIBN) as initiator and the polymers' properties were characterized. Then the synthesized MIP was employed as recognition element by packing into flow cell to establish a novel flow injection CL sensor. The CL intensity responded linearly to the concentration of Phe in the range 1.3 x 10-6 to 5.44 x 10-4 mol/L with a detection limit of 6.23 x 10-7 mol/L (3-σ), which is lower than that of conventional methods. The sensor is reusable and has a great improvement in sensitivity and selectivity for CL analysis. As a result, the new MIP-CL sensor had been successfully applied to the determination of Phe in samples
Template and target information: L-phenylalanine, Phe
Author keywords: molecularly imprinted polymeric microspheres, flow injection, chemiluminescence, phenylalanine


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