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Abstract: In this research, a novel strategy was developed to prepare molecularly imprinted polymer (MIP) coated solid-phase microextraction fibers on a large scale with Sudan I as template and stainless steel fibers as substrate. More than 20 fibers could be obtained in one glass tube, and the efficiency and coating repeatability were enhanced remarkably in contrast with the yield of only one fiber in our previous works. The obtained MIP-coated stainless steel fibers were characterized by homogeneous and highly cross-linked coating, good chemical and thermal stabilities, high extraction capacities, and specific selectivities to Sudan I - IV dyes. Based on the systemic optimization of extraction conditions, a simple and cost-effective method based on the coupling of MIP-coated SPME with high-performance liquid chromatography was developed for the fast and selective determination of trace Sudan I - IV dyes in hot chili powder and poultry feed samples. The limits of detection of Sudan I - IV dyes were within 2.5 - 4.6 ng g-1, and the spiked recoveries were in the range of 86.3 - 96.3% for hot chili powder sample and 84.6 - 97.4% for poultry feed sample
Template and target information: Sudan dyes, Sudan I, Sudan II, Sudan III, Sudan IV
Author keywords: molecularly imprinted polymer, solid-phase microextraction, Fiber preparation, Sudan dyes