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Reference type: Journal
Authors: Liu N, Zhao ZG, Chen YP, Gao ZX
Article Title: Rapid Detection of Staphylococcal Enterotoxin B by Two-Dimensional Molecularly Imprinted Film-Coated Quartz Crystal Microbalance.
Publication date: 2012
Journal: Analytical Letters
Volume: 45
Issue: (2-3)
Page numbers: 283-295.
DOI: 10.1080/00032719.2011.633186

Abstract: We fabricated a two-dimensional (2D) molecularly imprinted sol-gel thin film-coated quartz crystal microbalance (QCM) for the rapid detection of staphylococcal enterotoxin B (SEB) by combining organosilanes and the template protein SEB on the surface of piezoelectric quartz crystal (PQC) Au-electrode by in-situ immobilization. The detection process was monitored by the QCM's frequency shift (Δ f). The working range of this method was 1.0 x 10-1 - 1.0 x 10 3 μg/mL. The detection limit was 6.1 ng/mL, which was lower than that of the PQC immunosensor, and the detection period was within 0.5 h. The reproducibility of the imprinted film-coated QCM was satisfactory due to no significant statistical difference (P > 0.05) in the rapid detection of SEB between intra- and inter-batch. The selectivity of the imprinted sol-gel film showed that it could discriminate the template molecule from its analogues and other guest molecules. Compared with immunochip, the imprinted film-coated QCM is more advantageous in terms of simplicity, rapidity, low cost, and sensitivity. Moreover, in real sample analysis, the recoveries of this method were 89.4 - 106.63%, which can be considered a favorable and applicable method for the rapid determination of SEB in real samples
Template and target information: staphylococcal enterotoxin B, SEB
Author keywords: molecularly imprinted, Piezoelectric quartz crystal (PQC), quartz crystal microbalance (QCM), Staphylococcal enterotoxin B (SEB)


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