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Abstract: In this study, a simple preparation approach was developed for modified bisphenol A (BPA) molecularly imprinted polymer sorbent used in the hollow fiber solid phase microextraction (MIP-HF-SPME) of chlorogenic acid (CGA). The pre-polymer solution containing the template was introduced into the polypropylene hollow fiber segment for in situ polymerization. MIP-HF-SPME conditions based on the modified MIP-sorbent were optimized. Finally, the tool was used for selective extraction of chlorogenic acid in Echinacea purpurea, a medicinal plant. Main parameters affecting synthesis of organic-inorganic hybrid MIP and microextraction procedure were investigated and optimized. The measurements were done under the optimal conditions. The limit of detection has been gained 0.08 ng/mL. The linear range and relative standard deviation (RSD %) are 0.2 - 1000 ng/mL and 0.38 (n = 3) respectively. The average relative recoveries of spiked analyte in the four concentration levels were between 84.8 and 97.2%
Template and target information: bisphenol A, BPA, chlorogenic acid, CGA
Author keywords: chlorogenic acid, Hollow fiber solid phase microextraction, Molecularly imprinted polymers