Abstract: In this study, an electrochemically enhanced solid-phase microextraction (EE-SPME) approach based on molecularly imprinted polypyrrole/multi-walled carbon nanotubes (MIPPy/MWCNTs) composite coating on Pt wire was developed for selective extraction of fluoroquinolone antibiotics (FQs) in aqueous samples. During the extraction, a direct current potential was applied to the MIPPy/MWCNTs/Pt fiber as working electrode in a standard three-electrode system, FQ ions suffered electrophoretic transfer to the coating surface and then entered into the shape-complimentary cavities by hydrogen-bonding and ion-exchange interactions. After EE-SPME extraction, the fiber was desorbed with desorption solvent for high-performance liquid chromatography (HPLC) analysis. Some parameters influencing EE-SPME extraction such as applied potential, extraction time, solution pH, ionic strength, and desorption solvent were optimized. EE-SPME showed good selectivity and higher extraction efficiency to FQs compared with that of traditional solid-phase microextraction. EE-SPME coupled with HPLC to determine FQs in water samples, the limits of detection (S/N = 3) for the selected FQs are 0.5 - 1.9 μg L-1. The proposed method was successfully used to the analysis of FQs spiked urine and soil samples, with recoveries of 85.1 - 94.2% for the urine samples and 89.8 - 95.5% for the soil samples.
Template and target information: fluoroquinolone antibiotics, FQs, Gatifloxacin, GAT, balofloxcacin, BAL, enrofloxacin, ENR, norfloxacin, NOR, sarafloxacin, SAR
Author keywords: solid-phase microextraction, molecularly imprinted polymer, Fluoroquinolones, high-performance liquid chromatography, polypyrrole