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Reference type: Journal
Authors: Zhang M, Zhang XH, He XW, Chen LX, Zhang YK
Article Title: A self-assembled polydopamine film on the surface of magnetic nanoparticles for specific capture of protein.
Publication date: 2012
Journal: Nanoscale
Volume: 4
Issue: (10)
Page numbers: 3141-3147.
DOI: 10.1039/C2NR30316G

Abstract: In this study, we report a facile method for the preparation of core-shell magnetic molecularly imprinted polymers (MIPs) for protein recognition. Uniform carboxyl group functionalized Fe3O4 nanoparticles (NPs) were synthesized using a solvothermal method. Magnetic MIPs were synthesized by self-polymerization of dopamine in the presence of template protein on the surface of the Fe3O4 NPs. A thin layer of polydopamine can be coated on Fe3O4 NPs via dopamine self-polymerization and the imprinted polydopamine shells can be controlled by the mass ratio of Fe3O4 NPs and dopamine. More importantly, there is a critical value of polydopamine shell thickness for the maximum rebinding capacity. The as-prepared lysozyme-imprinted Fe3O4@polydopamine NPs show high binding capacity and acceptable specific recognition behavior towards template proteins. This method provides the possibility for the separation and enrichment of abundant proteins in proteomic analysis
Template and target information: protein, lysozyme


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