MIPs logo MIPdatabase      Use this space
Custom Search
Reference type: Journal
Authors: Yamazaki T
Article Title: An Amperometric Sensor Based on Gold Electrode Modified by Soluble Molecularly Imprinted Catalyst for Fructosyl Valine.
Publication date: 2012
Journal: Electrochemistry
Volume: 80
Issue: (5)
Page numbers: 353-357.
DOI: 10.5796/electrochemistry.80.353

Abstract: An amperometric sensor based on a soluble molecularly imprinted catalyst (MIC) has been developed for the detection of fructosyl amine compounds. A soluble MIC containing water-soluble functional monomers, an imidazole catalyst, and small amounts of a hydrophilic cross-linker is developed and used as a fructosyl amine oxidase mimic and for amperometric sensor construction. Fructosyl valine (Fru-val), a model compound of glycated hemoglobin, HbA1c, is used as the template. The MIC specifically oxidizes Fru-val in the presence of 1-methoxyphenazine methosulfate (electron acceptor) and reacts with the glycated peptide, fructosyl-valine-histidine sequence at the N-terminal of the beta-globin in HbA1c. The biosensor was fabricated by immobilizing the soluble MIC on Au electrodes via 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)-mediated amidation coupling. Using the soluble MIC-based sensor, 0.05 to 0.6 mM Fru-val could be determined at 40 degrees C and neutral pH. The steady-state current increase for this sensor was 33 nA in the presence of 0.05 mM Fru-val. The sensor showed 1.4 times higher sensitivity to Fru-val than to Fru-epsilon-lys, the competitor in HbA1c detection. (C) The Electrochemical Society of Japan, All rights reserved
Template and target information: fructosyl amine, Fru-val, fructosyl valine
Author keywords: Molecularly imprinted catalyst, amperometric sensor, Fructosyl valine, glycated hemoglobin


  Aluminium periodic table shirt  Scientist peptide mug  I shop perodically customisable tote bag

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner