Abstract: OBJECTIVE:To explore the preparation methods of drug-molecularly imprinted polymer in aqueous phase and the substrate-recognition behaviors of the polymers. METHODS:The β-blocked reagents carvedilol and metoprolol molecularly imprinted polymers were successfully synthesized in aqueous phase by utilizing the polymerisable ß-cyclodextrin as functional monomer, N,N-methylene-bisacrylamide as crosslinker, respectively. The affinity and selectivity of imprinted polymers for the templates and its structural analogues were evaluated in acetonitrile-water solvent by equilibrium binding experiments. RESULTS:The carvedilol and metoprolol molecularly imprinted polymers exhibited higher substrate-selectivity and recognition ability toward template, and the adsorption behaviors of the imprinted polymers were same as the "key-lock" relation between the enzyme and the substrate, however, the blank polymer didn't. CONCLUSIONS:The studies provided a feasible way to prepare molecularly imprinted polymers for drugs molecules in aqueous phase with highly recognizing ability.
Template and target information: carvedilol, metoprolol
Author keywords: molecularly imprinted polymer, β-cyclodextrin, Carvedilol, Metoprolol