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Reference type: Journal
Authors: Jing Z, Chao XL, Li C, Xiang XZ, Guang QX, Ming SJ
Article Title: Separation and determination of trace environmental estrogen through molecularly imprinted solid phase extraction coupled to high performance liquid chromatography.
Publication date: 2012
Journal: Journal of Environmental Science and Health, Part A
Volume: 47
Issue: (12)
Page numbers: 1889-1896.
DOI: 10.1080/03601234.2012.676438

Abstract: In recent years, there has been an increasing demand on the determination of trace environmental estrogen. In this study, a new 17β-estradiol molecularly imprinted polymer was successfully synthesized on the surface of activated silica gel. This prepared molecularly imprinted polymer was characterized, and found to exhibit good recognition, high selectivity, and a rapid adsorption-desorption dynamic toward 17β-estradiol. Using the molecularly imprinted polymer as a sorbent, an effective method of solid phase extraction coupled to high performance liquid chromatography for the separation and determination of 17β-estradiol was developed. Under optimal conditions, the detection limit (S/N = 3) of 17β-estradiol was 12.0 ng L-1. The enrichment factor was 198, and the linear range of the calibration graph was 0.05 - 300 mg L-1. Blank water samples spiked with 17β-estradiol at three different levels were analyzed by the developed method to afford recoveries ranging from 82.7% to 103.0%. Moreover, this presented method was applied to the quantitative detection of 17β-estradiol in a polluted water sample with a level of 0.024 mg L-1
Template and target information: 17β-estradiol
Author keywords: 17β-estradiol, separation, surface molecular imprinting, solid phase extraction, high performance liquid chromatography


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