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Reference type: Journal
Authors: Su QL, Zeng CY, Tang YW, Finlow DE, Cao M
Article Title: Evaluation of Diazepam-Molecularly Imprinted Microspheres for the Separation of Diazepam and its Main Metabolite from Body Fluid Samples.
Publication date: 2012
Journal: Journal of Chromatographic Science
Volume: 50
Issue: (7)
Page numbers: 608-614.
DOI: 10.1093/chromsci/bms039
Alternative URL: http://chromsci.oxfordjournals.org/content/50/7/608.abstract

Abstract: Molecularly imprinted microspheres (MIMs) for the drug diazepam and its main metabolite (nordiazepam) were prepared and used to separate the two species from urine and serum samples via molecularly imprinted solid-phase extraction. The specific binding capacity for diazepam was determined to be 1.97 mg/g, resulting in an imprinting factor of 5.8. The MIMs exhibit highly selective binding affinity for tricyclic benzodiazepines. Water-acetonitrile-acetone mixtures were used as the washing solvent and resulted in complete baseline separation, with a recovery of >87% for diazepam and of 88% for nordiazepam. The limits of detection are 21.5 and 24.5 ng/mL, respectively
Template and target information: diazepam, nordiazepam

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