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Reference type: Journal
Authors: Minami K, Ihara M, Kuroda S, Tsuzuki H, Ueda H
Article Title: Open-Sandwich Molecular Imprinting: Making a Recognition Matrix with Antigen-Imprinted Antibody Fragments.
Publication date: 2012
Journal: Bioconjugate Chemistry
Volume: 23
Issue: (7)
Page numbers: 1463-1469.
DOI: 10.1021/bc3000782

Abstract: A novel antibody-polymer conjugation method termed open-sandwich molecular imprinting (OS-MIP) has been proposed to produce a specific recognition matrix in the presence of a target antigen. The resultant carboxymethyldextran matrix conjugated with two separate antibody variable region fragments imprinted with the cognate antigen showed higher antigen-binding capacity than non-imprinted ones and was successfully used to sensitively monitor multiple antigen binding/desorption events by a surface plasmon resonance biosensor. Furthermore, when each fragment was labeled with different fluorophores before conjugation, the fluorescence signals of the matrix made by OS-MIP clearly showed an antigen concentration dependent increase in Förster resonance energy transfer between the two dyes. By using a combination of various methods for detecting interaction, OS-MIP will be a useful platform for detecting various targets from small molecules to proteins with high sensitivity and specificity
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