MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Adrus N, Ulbricht M
Article Title: Molecularly imprinted stimuli-responsive hydrogels for protein recognition.
Publication date: 2012
Journal: Polymer
Volume: 53
Issue: (20)
Page numbers: 4359-4366.
DOI: 10.1016/j.polymer.2012.07.062
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0032386112006453

Abstract: Temperature-responsive poly(N-isopropylacrylamide)-based (PNIPAAm) hydrogels were imprinted with lysozyme via in situ photo-initiated crosslinking polymerization. The three-dimensional network of the hydrogels was tailored by tuning the ionic content through methacrylic acid as template-binding comonomer while keeping the ratio between crosslinker (N,N'-methylenebisacrylamide) and N-isopropylacrylamide fixed. Moderate salt concentrations (0.3 m NaCl) were found to be suited for template removal without phase separation of the hydrogel. Swelling and protein (lysozyme and cytochrome C) binding were investigated for imprinted and nonimprinted gels at temperatures below and above the lower critical solution temperature of PNIPAAm (32 C). Imprinted gels showed a much higher affinity, selectivity and binding capacity for lysozyme compared to the nonimprinted reference materials. Protein binding capacity was strongly reduced above 32 C, to zero for nonimprinted and to small values for imprinted gels. Most important, specific lysozyme binding to the imprinted gels caused a large concentration dependent deswelling. This effect was much smaller for nonimprinted gels, and the response could be modulated by the content of the comonomer methacrylic acid. Overall, this approach is interesting for the development of novel sensors or materials for controlled release applications
Template and target information: protein, lysozyme
Author keywords: hydrogel, molecular imprinting, photopolymerization


  I love MISPE shirt  multi MIPs logo mug    Shirt featuring the template Lysozyme

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner